Karyotypic characterization of Hydromedusa tectifera (Testudines, Pleurodira) from the upper Iguaçu River in the Brazilian state of Paraná

Genetics and Molecular Biology

Rafael Bueno Noleto²  Daniel Luis Zanella Kantek²  Ana Cláudia Swarça¹  Ana Lúcia Dias¹  Alberto Sergio Fenocchio¹  Marta Margarete Cestari² 

[1] ,Universidade Federal do Paraná Departamento de Genética Curitiba PR ,Brazil

关键词: chromosomes;    FISH;    G-banding;    pleurodira;    turtle;   

DOI  :  10.1590/S1415-47572006000200011

来源: SciELO

【 摘 要 】

We present the karyotypic characterization of 26 specimens of the side-necked turtle Hydromedusa tectifera collected in the upper Iguaçu River, Paraná state, Brazil. The turtles were cytogenetically analyzed using Giemsa staining and other banding techniques (C, G, Ag-NOR and CMA3) as well as fluorescence in situ hybridization (FISH) with a rDNA 18S probe. All the specimens showed a diploid number of 58 composed of 22 macro and 36 microchromosomes. The Ag-NOR, CMA3 and FISH techniques permitted the identification and characterization of the chromosome pairs bearing nucleolus organizer regions (NORs), while G-banding facilitated a better recognition and pairing of macrochromosomes. These data agree with some information available in the literature and should be very useful for further cytotaxonomic and cytosystematic studies.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License