Comparison of I-FISH and G-banding for the detection of chromosomal abnormalities during the evolution of myelodysplastic syndrome

Brazilian Journal of Medical and Biological Research

R.f. Pinheiro¹  M.l.l.f. Chauffaille¹ 

[1] ,Universidade Federal de São Paulo Escola Paulista de Medicina Disciplina de Hematologia e HemoterapiaSão Paulo SP ,Brasil

关键词: Myelodysplastic syndrome;    FISH;    G-banding;   

DOI  :  10.1590/S0100-879X2009001100018

来源: SciELO

【 摘 要 】

Myelodysplastic syndrome (MDS) patients with a normal karyotype constitute a heterogeneous group from a biological standpoint and their outcome is often unpredictable. Interphase fluorescence in situ hybridization (I-FISH) studies could increase the rate of detection of abnormalities, but previous reports in the literature have been contradictory. We performed I-FISH and conventional karyotyping (G-banding) on 50 MDS patients at diagnosis, after 6 and 12 months or at any time if a transformation to acute myeloid leukemia (AML) was detected. Applying a probe-panel targeting the centromere of chromosomes 7 and 8, 5q31, 5p15.2 and 7q31, we observed one case with 5q deletion not identified by G-banding. I-FISH at 6 and 12 months confirmed the karyotype results. Eight cases transformed to AML during follow-up, but no hidden clone was detected by I-FISH in any of them. The inclusion of I-FISH during follow-up of MDS resulted in a small improvement in abnormality detection when compared with conventional G-banding.

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