Use of the TSPY gene for sexing cattle

Genetics and Molecular Biology

Daniela Cristina Lemos¹  Álvaro Fabrício Lopes Rios¹  Lisandra Cristina Caetano¹  Raysildo Barbosa Lôbo¹  Reginaldo Aparecido Vila¹  Lúcia Martelli¹  Paula Lumy Takeuchi¹  Ester Silveira Ramos¹ 

[1] ,Universidade de São Paulo Faculdade de Medicina de Ribeirão Preto Departamento de GenéticaRibeirão Preto SP ,Brazil

关键词: bovine;    cattle;    PCR;    sexing;    TSPY;   

DOI  :  10.1590/S1415-47572005000100020

来源: SciELO

【 摘 要 】

The Y-encoded, testis-specific protein (TSPY) is a Y-specific gene. The copy numbers of TSPY range from 20 to 60 in men and up to 200 in bulls. In this study, we examined the possibility of using the TSPY gene to sex cattle. DNA from blood samples of 100 Nelore cattle (50 males and 50 females) from the Nelore Cattle Breeding Program (PMGRN) was screened for TSPY by PCR using TSPY-specific primers. The assay was highly specific since all male samples were TSPY-positive and all female samples were negative. Positive results were also obtained at low DNA concentrations (less than 1 rhog/muL). These results showed that TSPY was a good male-specific marker, the usefulness of which was enhanced by the high copy number of the gene. This is the first report to demonstrate the applicability of TSPY for sexing cattle.

【 授权许可】

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