Species‐specific PAMP recognition by TLR2 and evidence for species‐restricted interaction with Dectin‐1

Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society

关键词: cattle;    bovine;    human;    toll‐;    like receptors;    C‐;    type lectin receptors;   

DOI  :  10.1189/jlb.0812390

学科分类：生理学

来源: Federation of American Societies for Experimental Biology

【 摘 要 】

TLRsmediaterecognitionofawiderangeofmicrobialproducts,includingLPS,lipoproteins,flagellin,andbacterialDNA,andsignalingthroughTLRsleadstotheproductionofinflammatorymediators.InadditiontoTLRs,manyothersurfacereceptorshavebeenproposedtoparticipateininnateimmunityandmicrobialrecognition,andsignalingthroughsomeofthese,forexample,C‐typelectins,islikelytocooperatewithTLRsignalingindefininginflammatoryresponses.Inthepresentstudy,weexaminedtheimportanceoftheECDandintracellularTIRdomainofboTLR2andhuTLR2toinduceaspecies‐specificresponsebycreatingachimericTLR2protein.OurresultsindicatethatthestrengthoftheresponsetoanyTLR2ligandtestedwasdependentontheextracellular,solenoidstructure,butnottheintracellularTIRdomain.Furthermore,weexaminedwhethertherecognitionoftwoPAMPsbyDectin‐1,aCLR,dependsontheinteractionwithTLR2fromthesamespecies.TLR2expressionseemedtoaffecttheDectin‐1‐dependentproductionofCXCL8toβ‐glucancontainingzymosanaswellasListeriamonocytogenes.Furthermore,theinteractionofDectin‐1withTLR2seemedtorequirethatbothreceptorsarefromthesamespecies.OurdatademonstratethatthedifferencesintheTLR2responseseenbetweenthebovineandhumansystemdependontheECDofTLR2andthatcollaborativerecognitionofdistinctmicrobialcomponentsbydifferentclassesofinnate‐immunereceptorsiscrucialinorchestratinginflammatoryresponses...

【 授权许可】

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