Genetic Diagnosis of Band 3 Deficiency and Sexing in Bovine Preimplantation Embryos

Journal of Veterinary Medical Science

Sadao ONOE¹  Akira MINAMIHASHI¹  Mutsumi INABA²  Daisuke ITO²  Soichi KAGEYAMA¹  Hiroki HIRAYAMA¹  Hiroshi OHTA²  Ken SAWAI¹  Satoru MORIYASU¹ 

[1] Hokkaido Animal Research Center;Laboratory of Molecular Medicine, Graduate School of Veterinary Medicine, Hokkaido University

关键词: band 3 deficiency;    cattle;    embryo;    genetic diagnosis;    sexing;   

DOI  :  10.1292/jvms.68.319

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(15)Cited-By(3)Band 3 deficiency with hereditary spherocytosis and hemolytic anemia in Japanese black cattle, band 3Bov.Yamagata, is caused by a total lack of band 3 protein with an autosomal dominant inheritance. Genotyping for band 3 deficiency and sexing were successfully achieved in biopsied embryo cells with efficiencies of 98.4% and 97.4%, respectively. Transfer of the embryo that was determined as homozygous for the mutant allele into a recipient cow resulted in the production of a fetus exhibiting the genotype and red cell phenotypes characteristic of band 3Bov.Yamagata. These results demonstrate that our procedure is reliable and applicable to produce animals free from or homozygous for the mutant allele by breeding carrier animals.

【 授权许可】

Unknown