Expression of the bacterial recA gene impairs genetic recombination and sporulation in a Saccharomyces cerevisiae diploid strain

Genetics and Molecular Biology

Marcos Antonio De Morais Junior²  José Ferreira Dos Santos²  João Antonio Pegas Henriques¹ 

[1] ,Universidade Federal de Pernambuco Departamento de Genética Recife PE ,Brazil

关键词: DNA repair;    genetic recombination;    recA gene;    sporulation;    yeast;   

DOI  :  10.1590/S1415-47572003000200016

来源: SciELO

【 摘 要 】

The Escherichia coli RecA protein (RecAp) has been demonstrated to induce mutagenesis in yeast cells, although there is still little information on the role of the RecAp in yeast recombination events. We evaluated spontaneous and induced general recombination in vegetative and meiotic cells of the XS2316 strain of the yeast Saccharomyces cerevisiae bearing the recA gene. We found that RecAp decreased reciprocal recombination, gene conversion and intrachromosomal recombination and promoted an increase in error-prone processes in both vegetative and meiotic cells, while its negative effect on meiotic recombination blocked ascospore formation.

【 授权许可】

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