Classical and molecular cytogenetic analysis in head and neck squamous cell carcinomas

Genetics and Molecular Biology

Luciana Cs Veiga²  Nádia A Bérgamo²  Luiz Paulo Kowalski¹  Silvia R Rogatto¹ 

[1] ,UNESP Instituto de Biociências Departamento de GenéticaBotucatu SP ,Brazil

关键词: FISH;    chromosomal aberrations;    head and neck cancer;    chromosome 17;   

DOI  :  10.1590/S1415-47572003000200003

来源: SciELO

【 摘 要 】

Head and neck carcinomas represent the sixth most frequent type of cancer in the world, and 90% are derived from squamous cells (HNSCC). In this study of 15 HNSCC cases, extensive aneuploidy was detected by G banding in most tumors. The most frequently observed numerical changes involved gain of a chromosome 22, and loss of chromosomes Y, 10, 17, and 19. The most frequent structural alteration was del(22)(q13.1). As compared to G-banding, fluorescence in situ hybridization (FISH) proved to be an effective technique for detecting aneuploidy. Interphase FISH with a chromosome 17 centromere probe disclosed a high frequency of monosomy for chromosome 17, in contrast with G-banding, by which clonal monosomy 17 was detected in only three of the tumors. Painting probes for chromosomes 5 and 16 were used to evaluate a selected series of HNSCC in which G-banding analysis had shown marker chromosomes. FISH analysis failed to confirm the origin of the marker chromosomes, but four out of five cases showed a significant loss of chromosomes 5. This difference between FISH and G-banding results may reflect the smaller number of metaphase analyzed as well as the criteria adopted for sorting these metaphases. Therefore results obtained solely by G-banding analysis should be considered with caution. Our data confirmed the involvement of chromosome 17 in head and neck squamous cell carcinomas.

【 授权许可】

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