Chromosome 17 abnormalities and mutation of the TP53 gene: correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia

Genetics and Molecular Biology

Luize Otero²  Geraldo Barroso Cavalcanti Júnior¹  Claudete Esteves Klumb¹  Marcos Antonio Mauricio Scheiner¹  Eliane Pereira Simões Magluta¹  Teresa De Souza Fernandez²  Maria Luiza Macedo Silva²  Virgínia Pires²  Gabriela Vasconcelos Andrade²  Raquel Ciuvalschi Maia¹  Daniel Tabak² 

[1] ,Instituto Nacional de Câncer Centro de Transplante de Medula Óssea Rio de Janeiro RJ ,Brazil

关键词: chronic myeloid leukemia;    chromosome 17;    TP53 gene;    p53 protein;   

DOI  :  10.1590/S1415-47572005000100007

来源: SciELO

【 摘 要 】

chronic myeloid leukemia (CML) have been described. This chromosomal region contains the tumor suppressor gene TP53 that may be an important factor in the evolution of this disease. In this study, we used flow cytometry and western blotting to assess p53 protein expression and single stranded conformational polymorphism to examine TP53 gene alterations in three patients with CML who showed alterations in 17p. Only the case with del(17)(p11) had p53 expression positive by flow cytometry and an abnormal migration pattern by SSCP analysis. The importance of the correlation between the results obtained with these techniques, as well as the clinical course of the patients, are discussed.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License