Magnetic bead technology for viral RNA extraction from serum in blood bank screening

Brazilian Journal of Infectious Diseases

Guilherme Ambrozio Albertoni²  Carine Prisco Arnoni²  Patricia Regina Barboza Araujo²  Sheila Siqueira Andrade²  Fabrício Oliveira Carvalho²  Manoel João Batista Castello Girão²  Nestor Schor¹  José Augusto Barreto² 

[1] ,Associação Beneficente de Coleta de SangueSão Paulo SP ,Brazil

关键词: HCV;    HIV;    infection;    reverse transcriptase polymerase chain reaction;    blood banks;   

DOI  :  10.1590/S1413-86702011000600008

来源: SciELO

【 摘 要 】

Nucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. OBJECTIVE: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase - polymerase chain reaction (RT-PCR) one-step. METHODS: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR. RESULTS: Out of 20.808 samples screened, 53 samples (29 for HCV and 24 for HIV) were confirmed as positive by serological and NAT methods. CONCLUSION: The manual magnetic bead-based extraction in combination with real-time PCR detection can be used to routinely screen blood donation for viremic donors to further increase the safety of blood products.

【 授权许可】

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