Magnetic bead technology for viral RNA extraction from serum in blood bank screening

Brazilian Journal of Infectious Diseases

Albertoni, Guilherme Ambrozio¹  Carvalho, Fabrício Oliveira¹  Girão, Manoel João Batista Castello¹  Schor, Nestor²  Arnoni, Carine Prisco¹  Barreto, José Augusto¹  Araujo, Patricia Regina Barboza¹  Andrade, Sheila Siqueira¹ 

[1] Associação Beneficente de Coleta de Sangue, São Paulo, Brazil;Universidade Federal de São Paulo, Brazil

关键词: HCV;    HIV;    infection;    reverse transcriptase polymerase chain reaction;    blood banks;   

DOI  :  10.1590/S1413-86702011000600008

来源: Contexto

【 摘 要 】

Nucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. OBJECTIVE: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase - polymerase chain reaction (RT-PCR) one-step. METHODS: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR.RESULTS: Out of 20.808 samples screened, 53 samples (29 for HCV and 24 for HIV) were confirmed as positive by serological and NAT methods.CONCLUSION: The manual magnetic bead-based extraction in combination with real-time PCR detection can be used to routinely screen blood donation for viremic donors to further increase the safety of blood products.

【 授权许可】

CC BY-NC-ND