GROWTH AND COMPOSITION OF Arthrospira (Spirulina) platensis IN A TUBULAR PHOTOBIOREACTOR USING AMMONIUM NITRATE AS THE NITROGEN SOURCE IN A FED-BATCH PROCESS

Brazilian Journal of Chemical Engineering

C. Cruz-martínez¹  C. K. C. Jesus¹  M. C. Matsudo¹  E. D. G. Danesi¹  S. Sato¹  J. C. M. Carvalho¹ 

关键词: Arthrospira (Spirulina) platensis;    Fed-batch cultivation;    Ammonium nitrate;    Light intensity;    Tubular photobioreactor;   

DOI  :  10.1590/0104-6632.20150322s00003062

来源: SciELO

【 摘 要 】

AbstractNH4NO3 simultaneously provides a readily assimilable nitrogen source (ammonia) and a reserve of nitrogen (nitrate), allowing for an increase in Arthrospira platensis biomass production while reducing the cost of the cultivation medium. In this study, a 22plus star central composite experimental design combined with response surface methodology was employed to analyze the influence of light intensity (I) and the total amount of added NH4NO3 (Mt) on a bench-scale tubular photobioreactor for fed-batch cultures. The maximum cell concentration (Xm), cell productivity (PX) and biomass yield on nitrogen (YX/N) were evaluated, as were the protein and lipid contents. Under optimized conditions (I = 148 μmol·photons·m-2·s-1 and Mt = 9.7 mM NH4NO3), Xm = 4710 ±34.4 mg·L-1, PX = 478.9 ±3.8 mg·L-1·d-1 and YX/N = 15.87 ±0.13 mg·mg-1 were obtained. The best conditions for protein content in the biomass (63.2%) were not the same as those that maximized cell growth (I = 180 μmol·photons·m-2·s-1 and Mt = 22.5 mM NH4NO3). Based on these results, it is possible to conclude that ammonium nitrate is an interesting alternate nitrogen source for the cultivation of A. platensisin a fed-batch process and could be used for other photosynthetic microorganisms.

【 授权许可】

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