Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey

Brazilian Journal of Chemical Engineering

R. Rech¹  M. A. Z. Ayub¹ 

[1] ,Federal University of Rio Grande do Sul State Food Science and Technology Institute Porto Alegre RS ,Brazil

关键词: Recombinant Saccharomyces cerevisiae;    beta-galactosidase;    Cheese-whey;    Fed-batch cultivation;   

DOI  :  10.1590/S0104-66322006000400001

来源: SciELO

【 摘 要 】

Saccharomyces cerevisiae strain W303 was transformed with two yeast integrative plasmids containing Kluyveromyces lactis LAC4 and LAC12 genes that codify beta-galactosidase and lactose permease respectively. The BLR030 recombinant strain was selected due to its growth and beta-galactosidase production capacity. Different culture media based on deproteinized cheese whey (DCW) were tested and the best composition (containing DCW, supplemented with yeast extract 1 %, and peptone 3 % (w/v)) was chosen for bioreactor experiments. Batch, and fed-batch cultures with linear ascending feeding for 25 (FB25), 35 (FB35), and 50 (FB50) hours, were performed. FB35 and FB50 produced the highest beta-galactosidase specific activities (around 1,800 U/g cells), and also the best productivities (180 U/L.h). Results show the potential use of fed-batch cultures of recombinant S. cerevisiae on industrial applications using supplemented whey as substrate.

【 授权许可】

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