Simultaneous use of sodium nitrate and urea as nitrogen sources improves biomass composition of Arthrospira platensis cultivated in a tubular photobioreactor

Engineering in Life Sciences

Ana Lucía Morocho‐Jácome¹  Laís deLara Capurro Guimarães¹  Camila Knysak Camargo deJesus¹  João Carlos Monteiro deCarvalho¹  Sunao Sato¹ 

[1] Department of Biochemical and Pharmaceutical Technology Faculty of Pharmaceutical Sciences, University of São Paulo São Paulo Brazil;

关键词: Arthrospira (Spirulina) platensis;    Biomass composition;    Sodium nitrate;    Tubular photobioreactor;    Urea;   

DOI  :  10.1002/elsc.201500051

来源: DOAJ

【 摘 要 】

Arthrospira platensis is widely cultivated in open ponds for industrial purposes. However, high‐protein A. platensis biomass produced in photobioreactors (PBRs) is recommended for pharmaceutical and cosmetic formulations. A. platensis was cultivated in a 3.5 L tubular airlift PBR using both sodium nitrate and urea as nitrogen sources. Sodium nitrate was added from the start of the cultivation using a batch process. Urea was supplied daily at exponentially increasing feeding rate using a fed‐batch process. The simultaneous optimization of the independent variables, namely, total quantity of sodium nitrate (mT1) and total quantity of urea (mT2), led to an optimal condition of mT1 = 15.0 mmol/L and mT2 = 7.5 mmol/L. Maximum biomass concentration (5183 ± 94 mg/L) corresponding to the highest biomass productivity (683 ± 13 mg/L/day) was obtained under such condition. The addition protocol of both nitrogen sources resulted in high productivities of protein (6.2 ± 0.4 mg/L/day) as well as chlorophyll‐a (372.2 ± 7.7 mg/L/day). Such innovative process could be applied in the large‐scale production of A. platensis using tubular PBR for novel applications.

【 授权许可】

Unknown