Physico-chemical, spectroscopical and thermal characterization of biodiesel obtained by enzymatic route as a tool to select the most efficient immobilized lipase

Brazilian Journal of Chemical Engineering

G. A. M. Silva¹  P. C. M. Da Rós¹  L. T. A. Souza¹  A. P. O. Costa¹  H. F. De Castro¹ 

[1] ,University of São Paulo Engineering School of Lorena Lorena SP ,Brazil

关键词: Biodiesel;    Beef tallow;    Ethanol route;    Immobilized lipase;    Thermogravimetric;    Rheological properties;   

DOI  :  10.1590/S0104-66322012000100005

来源: SciELO

【 摘 要 】

Two microbial lipases from Burkholderia cepacia and Pseudomonas fluorescens were evaluated as catalysts for the enzymatic transesterification of beef tallow with ethanol and the most efficient lipase source was selected by taking into account the properties of the product to be used as fuel. Both lipases were immobilized on an epoxy silica-polyvinyl alcohol composite by covalent immobilization and used to perform the reactions under the following operational conditions: beef tallow-to-ethanol molar ratio of 1:9, 45ºC and 400 units of enzymatic activity per gram of fat. Products, characterized using Fourier Transform Infrared spectroscopy (FTIR), viscosimetry, thermogravimetry and ¹H NMR spectroscopy, suggested that the biodiesel sample obtained in the reaction catalyzed by Burkholderia cepacia lipase has the best set of properties for fuel usage.

【 授权许可】

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