A lactose fia-biosensor system for monitoring and process control

Brazilian Journal of Chemical Engineering

L. S. Ferreira²  J. O. Trierweiler¹  M. B. De Souza Jr²  R. O. M. Folly² 

[1] ,Federal University of Rio de Janeiro School of Chemistry Chemical Engineering DepartmentRio de Janeiro RJ ,Brazil

关键词: lactose;    biosensor;    flow injection analysis;    Kluyveromyces marxianus;   

DOI  :  10.1590/S0104-66322004000200021

来源: SciELO

【 摘 要 】

In this work an enzymatic lactose biosensor composed of the immobilized enzymes b-galactosidase and glucose oxidase was developed. Oxygen consumption during the reaction catalyzed by these enzymes was detected. The biosensor was integrated into an FIA (flow injection analysis) system that allows measurement of lactose on-line in less than three minutes. This biosensor was used to monitor lactose concentration during the production of b-galactosidase by the yeast Kluyveromyces marxianus from cheese whey. The sensor showed good stability after four months and after almost 7000 measurements had been performed. The analytical curve was linear in the range of lactose concentration from 1 to 30 g/L.

【 授权许可】

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