Breakdown of resistance in sweet pepper against Pepper yellow mosaic virus in Brazil

Scientia Agricola

Ricardo Gioria²  Renato De Souza Braga²  Renate Krause-sakate¹  Caroline Roullier¹  Daniel Dias Rosa¹  Monika Fecury Moura¹  José Alberto Caram De Souza-dias¹  Haiko Enok Sawazaki¹  Luis Eduardo Aranha Camargo¹  Jorge Alberto Marques Rezende¹ 

[1] ,Sakata Seed Sudamerica LtdaBragança Paulista SP ,Brasil

关键词: Potyvirus;    RT-PCR;    ELISA;    resistance gene;    Potyvirus;    RT-PCR;    ELISA;    gene de resistência;   

DOI  :  10.1590/S0103-90162009000200017

来源: SciELO

【 摘 要 】

Plants of Capsicum annuum cv. Magali R, resistant to Pepper yellow mosaic virus (PepYMV), which showed severe yellow mosaic, leaf malformation and stunting were observed during the 2003/04 growing season in Lins, São Paulo State, Brazil. Potyvirus-like particles observed in leaf sap from infected plants under the electron microscope reacted with an antiserum against PepYMV in PTA-ELISA. In addition to C. annuum cv. Magali R, this potyvirus also infected systemically the resistant C. annuum cv. Rubia R. The nucleotide sequence of part of the CP gene of this potyvirus shared 96-98% identity with that of other PepYMV isolates. The partial nucleotide sequence of the 3' NTR showed 94-96% identity with that of PepYMV. These data indicate that this potyvirus is a resistance-breaking isolate of PepYMV.

【 授权许可】

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