Prevalence of Lettuce mosaic virus - common strain on three lettuce producing areas from São Paulo State

Summa Phytopathologica

Ana Carolina Firmino²  Renate Krause-sakate²  Marcelo Agenor Pavan²  Norberto Da Silva²  Sérgio Minoru Hanai¹  Roberto Hiroto Anbo¹  Thomas Nietzsche¹  Olivier Le Gall¹ 

[1] ,Universidade Estadual Paulista Faculdade de Ciências Agronômicas Depto. de Produção Vegetal/Defesa FitossanitáriaBotucatu SP

关键词: Potyvirus;    LMV;    RT-PCR;    detection;    resistance breaking;    Potyvirus;    LMV;    RT-PCR;    detecção;   

DOI  :  10.1590/S0100-54052008000200009

来源: SciELO

【 摘 要 】

LMV is one of the most important pathogens of lettuce worldwide. Based on their ability to overcome the resistance genes mo1¹ and mo1² in lettuce, isolates can be divided in two types: LMV-Most, which can infect and are seed-borne in cultivars containing the mo1 gene and LMV-Common, which do not cause symptoms on these cultivars and are seed transmitted only in susceptible cultivars. To evaluate the occurrence of these two types of LMV isolates, a survey was carried out during 2002-2005 in three lettuce production areas from São Paulo State. Total RNA was used for the diagnosis of LMV isolates by RT-PCR using universal primers for the variable N-terminus of the capsid protein, in the 3' end of the genome. Positives samples were analyzed by a second RT-PCR using specifics primers for LMV-Most isolates designed to amplify a fragment from the central region (CI-VPg) of the genome. A total of 1362 samples showing mosaic symptoms were collected and 504 (37.29 %) were positives for LMV. On susceptible lettuce cultivars, LMV-Common was prevalent (77.3%). LMV-Most was found frequently associated with tolerant (mo1¹) lettuce cultivars. Susceptible cultivars correspond today for most of the area of lettuce production. So, despite the ability of LMV-Most isolates to overcome the resistance provided by the recessive mo1¹ gene, they are not prevalent in the conditions of São Paulo State.

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