Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples

Ciência Rural

Herica Makino¹  Valéria Régia Franco Sousa¹  Mahyumi Fujimori¹  Juliana Yuki Rodrigues¹  Alvaro Felipe Lima Ruy Dias¹  Valéria Dutra¹  Luciano Nakazato¹  Arleana Do Bom Parto Ferreira De Almeida¹ 

关键词: canine ehrlichiosis;    diagnosis;    nested PCR;    biological samples.;    erliquiose canina;    diagnóstico;    nested PCR;    amostra biológica.;   

DOI  :  10.1590/0103-8478cr20150090

来源: SciELO

【 摘 要 】

ABSTRACT: The objective of this study was to compare the DNA detection of Ehrlichia canis in blood and bone marrow to determine the prevalence of the agent in Várzea Grande, Mato Grosso. Blood samples and bone marrow from 80 dogs of both sexes, different breeds and age, were collected and processed for a cross-sectional study performed using nested PCR. Of the 80 dogs, 61 (76.3%) had E. canis DNA in one of the samples. The buffy coat was positive in 42 dogs (52.5%) and the bone marrow was positive in 33 (41.3%). There was no significant association between the positive biological samples of either the buffy coat or bone marrow and the presence or absence of clinical signs (P=0.49). No risk factor was associated with infection in the studied area. The bone marrow samples were efficient for the molecular diagnosis of canine ehrlichiosis, particularly when there was a negative blood sample, although infection was present.

【 授权许可】

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