PCR-based detection of Mycobacterium avium subsp.paratuberculosis infection in cattle in South Korea using fecalsamples

Journal of Veterinary Medical Science

Han Sang YOO¹  Hong-Tae PARK¹  Min-Kyoung SHIN¹  Hyun-Eui PARK¹  Yong-Il CHO² 

[1] Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea;Department of Animal Science & Technology, Sunchon National University, Suncheon 57922, Korea

关键词: diagnosis;    Korea;    Mycobacterium avium subsp. paratuberculosis;    nested PCR;    real-time PCR;   

DOI  :  10.1292/jvms.15-0271

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Mycobacterium avium subsp. paratuberculosis (MAP) isthe causative agent of bovine paratuberculosis (PTB). The first step in the control of PTBis the identification and isolation of sub-clinical fecal shedders from the herd. In thecurrent study, real-time and nested PCR targeting MAP-specific genetic elements(IS900 and ISMAP02) DNA isolated from fecal sampleswere used to detect MAP infection in cattle. Of the 1,562 fecal samples obtained from 37herds, regardless of diarrhea, 35 samples tested positive in bothIS900-targeted real-time and ISMAP02-targeted nestedPCR. At the herd level, 12 of the 37 herds were found to be positive for MAP. Detectionrates of the PCR tests were similar to those reported for ELISA-based methods. Theseresults suggest that PCR can be used to detect sub-clinical fecal shedders of MAP.

【 授权许可】

Unknown