Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples

Ciência Rural

Almeida, Arleana do Bom Parto Ferreira de¹  Dutra, Valéria¹  Fujimori, Mahyumi²  Nakazato, Luciano¹  Dias, Alvaro Felipe Lima Ruy¹  Makino, Herica¹  Sousa, Valéria Régia Franco¹  Rodrigues, Juliana Yuki² 

[1] Universidade Federal de Mato Grosso (UFMT, Cuiabá, Brazil;Cuiabá, Cuiabá, Brasil

关键词:  canine ehrlichiosis;    diagnosis;    nested PCR;    biological samples;   

DOI  :  10.1590/0103-8478cr20150090

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

:The objective of this study was to compare the DNA detection of Ehrlichia canis in blood and bone marrow to determine the prevalence of the agent in Várzea Grande, Mato Grosso. Blood samples and bone marrow from 80 dogs of both sexes, different breeds and age, were collected and processed for a cross-sectional study performed using nested PCR. Of the 80 dogs, 61 (76.3%) had E. canis DNA in one of the samples. The buffy coat was positive in 42 dogs (52.5%) and the bone marrow was positive in 33 (41.3%). There was no significant association between the positive biological samples of either the buffy coat or bone marrow and the presence or absence of clinical signs (P=0.49). No risk factor was associated with infection in the studied area. The bone marrow samples were efficient for the molecular diagnosis of canine ehrlichiosis, particularly when there was a negative blood sample, although infection was present.

【 授权许可】

Unknown