Colibacillosis in lambs is associated to type I heat-stable enterotoxin in a farm in São Paulo State, Brazil

Ciência Rural

Annelize Zambon Barbosa Aragão²  Marcelo Ananias Teocchi²  Maria Clara Duarte Fregolente²  Maria Silvia Viccari Gatti²  Alexandre Vaz Pires¹  Tomomasa Yano² 

[1] ,Universidade Estadual de Campinas (UNICAMP)Campinas SP ,Brasil

关键词: lambs;    STa;    colibacillosis;    Escherichia coli;    carneiro;    STa;    colibacilose;    Escherichia coli;   

DOI  :  10.1590/S0103-84782012005000018

来源: SciELO

【 摘 要 】

Twenty seven (48.2%) culture supernatants of 56 Escherichia coli isolated from diarrheic lamb feces (7 to 10 days old) in São Paulo State, Brazil, presented positive results to suckling mice assay (fluid accumulation) but none caused cytopathic effects on Vero and CHO cells, indicating that these strains did not produced LT or VT toxins. PCR assays showed that these 27 E. coli strains harbored estA, that codifies for STa, but not for stx1, stx2 or cnf genes. The positive STa strains were checked for genes that codify for F41, F17 and K99 fimbriae, wich are considered colonization factors in ETEC. Only F17 was detect in two samples (7.4%). Twelve of 27 STa positive carried hlyA gene and presented hemolytic activity in blood Agar. Presence of rotavirus was not detected among the diarrheic feces. These data suggests that STa must be an important diarrheagenic factor to small ruminants in São Paulo State.

【 授权许可】

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