Characterization of Escherichia coli Strains Obtained from Layer Chickens Affected with Colibacillosis in a Commercial Egg-Producing Farm

Journal of Veterinary Medical Science

Koichi OTSUKI¹  Azusa SOMEYA²  Toshiyuki MURASE² 

[1] Faculty of Technology, Kyoto Sangyo University;Laboratory of Veterinary Microbiology, Faculty of Agriculture, Tottori University

关键词: age;    colibacillosis;    Escherichia coli;    layer;   

DOI  :  10.1292/jvms.69.1009

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)Cited-By(6)The present study reports colibacillosis of layer chickens in a commercial egg-producing farm in western Japan. Three flocks of chicken at 18-21 weeks of age were affected during the initiation of egg lay. Postmortem examination revealed pericarditis, perihepatitis, airsacculitis, subcutaneous inguinal lesion, and injured cloaca. Escherichia coli was isolated from the lesions of the affected birds. Twenty-two of 26 E. coli isolates (84.6%) obtained from 18 birds in the 3 flocks showed pulsed-field gel electrophoresis (PFGE) patterns that were considered to be closely associated to each other and arbitrarily designated as pattern A. All the 22 isolates with the PFGE pattern A harbored the putative virulence genes, astA, iss, iucD, tsh, and cva/cvi. Additional 2 PFGE patterns (B and C) were also found in E. coli isolates obtained from the affected flocks and had the putative virulence genes in combinations different from those in the pattern A strains. The results suggested that certain E. coli virulence genes and host factors, such as initiation of egg lay may be associated with occurrence of colibacillosis.

【 授权许可】

Unknown