Restriction enzyme analysis of the chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro

Ciência Rural

Sergio Echeverrigaray²  Maria Tereza Vitral Carvalho¹  Eric Derbyshire¹ 

[1] ,Universidade de Caxias do Sul Instituto de Biotecnologia Departamento de Ciências BiológicasCaxias do Sul RS

关键词: chloroplast genome;    common bean;    restriction analysis;    genoma cloroplástico;    feijão;    análise de restrição;   

DOI  :  10.1590/S0103-84781996000300030

来源: SciELO

【 摘 要 】

The chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro was isola ted from chloroplasts obtained by descontiuous sucrose gradient centrifugation. The restriction analysis with the enzymes HindIII, EcoRI and BamHI and their combination, allowed to identified more than 20 fragments of 18 to 0.65kb. The size of Phaseolus vulgaris L. cp DNA was estimated in 140kb with the presence of a repeat sequence of about 22kb.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License