Restriction enzyme analysis of the chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro

Ciência Rural

Derbyshire, Eric¹  Echeverrigaray, Sergio²  Carvalho, Maria Tereza Vitral³ 

[1] Universidade de Caxias do Sul, Caxias do Sul;Universidade de São Paulo

关键词: chloroplast genome;    common bean;    restriction analysis;   

DOI  :  10.1590/S0103-84781996000300030

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

The chloroplast DNA of Phaseolus vulgaris L. vr. Rio Negro was isola ted from chloroplasts obtained by descontiuous sucrose gradient centrifugation. The restriction analysis with the enzymes HindIII, EcoRI and BamHI and their combination, allowed to identified more than 20 fragments of 18 to 0.65kb. The size of Phaseolus vulgaris L. cp DNA was estimated in 140kb with the presence of a repeat sequence of about 22kb.

【 授权许可】

CC BY-NC