Molecular and biological diversity of HIV-1 in Brazil

Memórias do Instituto Oswaldo Cruz

José Carlos Couto-fernandez²  Mariza Gonçalves Morgado¹  Jairo Ivo Dos Santos¹  Bernardo Galvão-castro¹ 

[1] ,Instituto Oswaldo Cruz Centro de Pesquisas Gonçalo Moniz Laboratório Avançado de Saúde PúblicaSalvador,Brasil

关键词: Brazilian HIV;    virulence;    restriction analysis;    viral diversity;   

DOI  :  10.1590/S0074-02761992000200013

来源: SciELO

【 摘 要 】

To determine the genomic polymorphism and biological properties present in HIV-1 Brazilian isolates, were analyzed five viral isolates obtained from patients residing in Rio de Janeiro (P1 and P5), São Paulo (P3) and Bahia (P2 and P4) states. For each viral isolate in vitro characteristics such as replication rate, syncytium-inducing capacity and cell death were observed in lymphoblastoid (H9, CEM and peripheral blood mononuclear cells) as well as monocytoid (U937) cells. In addition, the evaluation of the restriction fragment lenght polymorphism of these isolates was also performed using a panel of endonucleases such as Hind III, Bgl II, Sac I, Pst I, Kpn I and Eco RI. One of the isolates (P1), showed the highest phenotypic and genotypic divergence, when compared to others. The results found suggest a HIV heterogeneity in Brazil similar to that already described in other regions of the world.

【 授权许可】

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