Characterization and in vitro cytocompatibility of an acid-etched titanium surface

Brazilian Dental Journal

Daniel Rey De Carvalho²  Paulo Sérgio Perri De Carvalho¹  Osvaldo Magro Filho¹  José Daniel Biasoli De Mello¹  Márcio Mateus Beloti¹  Adalberto Luiz Rosa¹ 

[1] ,Catholic University of Brasília Dental School Department of Oral and Maxillofacial SurgeryBrasília DF ,Brazil

关键词: cytocompatibility;    cell culture;    osteoblast;    titanium;    surface treatment;   

DOI  :  10.1590/S0103-64402010000100001

来源: SciELO

【 摘 要 】

The aims of this study were to characterize the microstructure of a commercially pure titanium (cpTi) surface etched with HCl/H2SO4 (AE-cpTi) and to investigate its in vitro cytocompatibility compared to turned cpTi (T-cpTi). T-cpTi showed a grooved surface and AE-cpTi revealed a surface characterized by the presence of micropits. Surface parameters indicated that the AE-cpTi surface is more isotropic and present a greater area compared to T-cpTi. The oxide film thickness was similar between both surfaces; however, AE-cpTi presented more Ti and O and less C. Osteoblastic cell proliferation, alkaline phosphatase activity, and bone-like nodule formation were greater on T-cpTi than on AE-cpTi. These results show that acid etching treatment produced a surface with different topographical and chemical features compared to the turned one, and such surface modification affected negatively the in vitro cytocompatibility of cpTi as demonstrated by decreasing culture growth and expression of osteoblastic phenotype.

【 授权许可】

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