Oxidative nanopatterning of titanium surfaces promotes production and extracellular accumulation of osteopontin

Brazilian Dental Journal

Renan De Barros E Lima Bueno²  Patricia Adachi²  Larissa Moreira Spinola De Castro-raucci²  Adalberto Luiz Rosa²  Antonio Nanci¹  Paulo Tambasco De Oliveira² 

[1] ,USP - University of São Paulo Ribeirão Preto Dental School Ribeirão Preto SP ,Brazil

关键词: osteopontin;    titanium;    nanotopography;    cell culture;    osteoblast;   

DOI  :  10.1590/S0103-64402011000300001

来源: SciELO

【 摘 要 】

The bone-biomaterial interface has been characterized by layers of afibrillar extracellular matrix (ECM) enriched in non collagenous proteins, including osteopontin (OPN), a multifunctional protein that in bone controls cell adhesion and ECM mineralization. Physical and chemical aspects of biomaterial surfaces have been demonstrated to affect cell-ECM-substrate interactions. The present paper described the ability of oxidative nanopatterning of titanium (Ti) surfaces to control extracellular OPN deposition in vitro. Ti discs were chemically treated by a mixture of H2SO4/H2O2 for either 30 min [Nano(30') Ti] or 4 h [Nano(4h) Ti]. Non-etched Ti discs were used as control. Primary osteogenic cells derived from newborn rat calvarial bone were plated on control and etched Ti and grown under osteogenic conditions up to 7 days. High resolution scanning electron microscopy revealed that treated Ti discs exhibited a nanoporous surface and that areas of larger nanopits were noticed only for Nano(4h) Ti. Large extracellular OPN accumulation were detectable only for Nano(4h) Ti, which was associated with OPN-positive cells with typical aspects of migrating cells. At day 3, quantitative results in terms of areas of OPN labeling were as follows: Nano(4h) Ti > Nano(30') Ti > Control Ti. In conclusion, chemically nanostructured Ti surfaces may support the enhancement of endogenous extracellular OPN deposition by osteogenic cells in vitro depending on the etching time, a finding that should be taken into consideration in strategies to biofunctionalize implant surfaces with molecules with cell adhesion capacity.

【 授权许可】

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