Chemical Constituents of the Seeds of Raphanus sativus and their Biological Activity

Journal of the Brazilian Chemical Society

Ki Hyun Kim¹  Eunjung Moon¹  Seoung Rak Lee¹  Kyoung Jin Park¹  Sun Yeou Kim¹  Sang Un Choi¹  Kang Ro Lee¹ 

关键词: Raphanus sativus;    Brassicaceae;    cytotoxicity;    anti-inflammation;    Mosher’s method;   

DOI  :  10.5935/0103-5053.20150222

来源: SciELO

【 摘 要 】

As part of our ongoing search for bioactive constituents of natural Korean medicinal resources, a bioassay-guided fractionation and chemical investigation of the MeOH extract of Raphanus sativus (Brassicaceae) seeds resulted in the isolation and identification of fifteen compounds, including a new phenolic compound. The structure of the new compound was determined by extensive spectroscopic analysis and the Mosher's method. One of the compounds has been recently reported as a synthetic product. Some compounds showed moderate antiproliferative activities against the tumor cell lines A549, SK-OV-3, SK-MEL-2, and HCT-15 with IC50 values in the range of 5.62 to 28.88 μM. Moreover, the anti-neuroinflammatory activities of the isolates were determined by measuring the nitric oxide (NO) levels in the medium using murine microglia BV-2 cells. With exception of one specific compound, all the others inhibited the lipopolysaccharide (LPS)-stimulated NO production (IC50 values < 200 μM).

【 授权许可】

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