Chemical Constituents of the Seeds of Raphanus sativus and their Biological Activity

Journal of the Brazilian Chemical Society

Kim, Sun Yeou¹  Lee, Seoung Rak¹  Sungkyunkwan University, Suwon, Republic of Korea¹  Kim, Ki Hyun¹  Korea Research Institute of Chemical Technology, Deajeon, Republic of Korea¹  Moon, Eunjung¹  Gachon University, Incheon, Republic of Korea¹  Park, Kyoung Jin¹  Lee, Kang Ro¹  Charmzone Co. LTD., Wonju, Republic of Korea¹  Choi, Sang Un¹ 

关键词:  Raphanus sativus;    Brassicaceae;    cytotoxicity;    anti-inflammation;    Mosher’s method;   

DOI  :  10.5935/0103-5053.20150222

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

As part of our ongoing search for bioactive constituents of natural Korean medicinal resources, a bioassay-guided fractionation and chemical investigation of the MeOH extract of Raphanus sativus (Brassicaceae) seeds resulted in the isolation and identification of fifteen compounds, including a new phenolic compound. The structure of the new compound was determined by extensive spectroscopic analysis and the Mosher's method. One of the compounds has been recently reported as a synthetic product. Some compounds showed moderate antiproliferative activities against the tumor cell lines A549, SK-OV-3, SK-MEL-2, and HCT-15 with IC50 values in the range of 5.62 to 28.88 μM. Moreover, the anti-neuroinflammatory activities of the isolates were determined by measuring the nitric oxide (NO) levels in the medium using murine microglia BV-2 cells. With exception of one specific compound, all the others inhibited the lipopolysaccharide (LPS)-stimulated NO production (IC50 values < 200 μM).

【 授权许可】

Unknown