Uncertainty in the determination of glucose and sucrose in solutions with chitosan by enzymatic methods

Journal of the Brazilian Chemical Society

Berta N. Estevinho¹  Amélia Ferraz¹  Lúcia Santos¹  Fernando Rocha¹  Arminda Alves¹ 

关键词: uncertainty;    enzymatic methods;    glucose;    sucrose;    chitosan;   

DOI  :  10.5935/0103-5053.20130119

来源: SciELO

【 摘 要 】

The purpose of this work was to evaluate the applicability of two enzymatic methods to quantify glucose and sucrose in aqueous solutions with chitosan. The analytical methods were validated and the main parameters, as limit of detection, linearity range, precision and accuracy were determined. The global uncertainty for glucose enzymatic method was less than 10% for concentration levels between 700 and 2000 mg L-1, and for the sucrose enzymatic method, the global uncertainty showed values less than 3% for all concentration levels analyzed (100-1000 mg L-1). Despite the similarities of performances with respect to other analytical methods, the enzymatic method proved to be better than others, in particular high performance liquid-chromatography (HPLC), mainly if a great number of samples needs to be analyzed, allowing a quick result, and because the mucoadhesive properties of chitosan make difficult the HPLC analytical methodology, creating stability problems in the chromatographic column.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License