Validated method for the determination of Gemifloxacin in bulk, pharmaceutical formulations and human serum by RP-HPLC: in vitro applications

Journal of the Brazilian Chemical Society

Najma Sultana²  M. Saeed Arayne¹  Sana Shamim²  Mahwish Akhtar²  Somia Gul² 

[1] ,University of Karachi Faculty of Pharmacy Research Institute of Pharmaceutical Sciences Department of Pharmaceutical ChemistryKarachi,Pakistan

关键词: gemifloxacin;    HPLC;    human serum;    student's t-test;   

DOI  :  10.1590/S0103-50532011000500024

来源: SciELO

【 摘 要 】

An isocratic reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the determination of gemifloxacin in bulk, dosage formulations and human serum at 270 nm. Chromatographic separation was achieved on Purospher STAR C18 (250 × 4.6 mm, 5 µm) column using mobile phase methanol:water (90:10, v/v) adjusted pH 2.8 via phosphoric acid 85% having flow rate of 1.5 mL min-1 at ambient temperature. Calibration curves were linear over range of 5-100 µg mL-1 with a correlation coefficient 0.9998. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.015 and 0.045 µg mL-1, respectively. Intra and inter-run precision and accuracy results were 98.73-100.12% and then correlated through student'st-test. This method was further applied for in vitro interactions of gemifloxacin with essential and trace elements.

【 授权许可】

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