Simultaneous determination of gemifloxacin and diuretics in bulk, pharmaceutical dosage forms and human serum by RP-HPLC

Química Nova

Najma Sultana²  Sana Shamim²  Mahwish Akhtarand Somia Gul²  M. Saeed Arayne¹ 

[1] ,University of Karachi Research Institute of Pharmaceutical Sciences Faculty of Pharmacy,Pakistan

关键词: gemifloxacin;    diuretics;    HPLC;   

DOI  :  10.1590/S0100-40422010000700029

来源: SciELO

【 摘 要 】

An isocratic reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the simultaneous determination of gemifloxacin and diuretics (hydrochlorothiazide and furosemide) in bulk, dosage formulations and human serum at 232 nm. Chromatographic separation was achieved on Purospher Start C18 (250 mm x 4.6 mm, 5 µm) column using mobile phase, methanol: water: acetonitrile (70:25:5 v/v/v) adjusted to pH 3.0 via phosphoric acid 85% having flow rate of 0.8 mL min -1 at room temperature. Calibration curves were linear over range of 0.5-10 µg mL -1 with a correlation coefficient ± 0.999. LOD and LOQ were in the ranges of 0.75-2.56 µg mL -1. Intra and inter-run precision and accuracy results were 98.26 to 100.9.

【 授权许可】

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