Simultaneous determination of gemifloxacin and diuretics in bulk, pharmaceutical dosage forms and human serum by RP-HPLC

Química Nova

Shamim, Sana¹  University of Karachi, Pakistan¹  Arayne, M. Saeed¹  Gul, Mahwish Akhtarand Somia¹  Sultana, Najma¹ 

关键词: gemifloxacin;    diuretics;    HPLC.;   

DOI  :  10.1590/S0100-40422010000700029

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

An isocratic reversed phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the simultaneous determination of gemifloxacin and diuretics (hydrochlorothiazide and furosemide) in bulk, dosage formulations and human serum at 232 nm. Chromatographic separation was achieved on Purospher Start C18 (250 mm x 4.6 mm, 5 µm) column using mobile phase, methanol: water: acetonitrile (70:25:5 v/v/v) adjusted to pH 3.0 via phosphoric acid 85% having flow rate of 0.8 mL min -1 at room temperature. Calibration curves were linear over range of 0.5-10 µg mL -1 with a correlation coefficient ± 0.999. LOD and LOQ were in the ranges of 0.75-2.56 µg mL -1. Intra and inter-run precision and accuracy results were 98.26 to 100.9.

【 授权许可】

Unknown