Enzymatic kinetic resolution of (RS)-1-(Phenyl)ethanols by Burkholderia cepacia lipase immobilized on magnetic nanoparticles

Journal of the Brazilian Chemical Society

Lya P. Rebelo²  Caterina G. C. M. Netto¹  Henrique E. Toma¹  Leandro H. Andrade² 

[1] ,Laboratory of Fine Chemistry and Biocatalysis

关键词: superparamagnetic nanoparticles;    lipase;    transesterification catalysis;    enantiomeric resolution;   

DOI  :  10.1590/S0103-50532010000800019

来源: SciELO

【 摘 要 】

Lipase from Burkholderia cepacia immobilized on superparamagnetic nanoparticles using adsorption and chemisorption methodologies was efficiently applied as recyclable biocatalyst in the enzymatic kinetic resolution of (RS)-1-(phenyl)ethanols via transesterification reactions. (R)-Esters and the remaining (S)-alcohols were obtained with excellent enantiomeric excess (> 99%), which corresponds to a perfect process of enzymatic kinetic resolution (conversion 50%, E > 200). The transesterification reactions catalysed with B. cepacia lipase immobilized by the glutaraldehyde method showed the best results in terms of reusability, preserving the enzyme activity (conversion 50%, E > 200) for at least 8 successive cycles.

【 授权许可】

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