Journal of the Brazilian Chemical Society | |
Enzymatic kinetic resolution of (RS)-1-(Phenyl)ethanols by Burkholderia cepacia lipase immobilized on magnetic nanoparticles | |
Lya P. Rebelo2  Caterina G. C. M. Netto1  Henrique E. Toma1  Leandro H. Andrade2  | |
[1] ,Laboratory of Fine Chemistry and Biocatalysis | |
关键词: superparamagnetic nanoparticles; lipase; transesterification catalysis; enantiomeric resolution; | |
DOI : 10.1590/S0103-50532010000800019 | |
来源: SciELO | |
【 摘 要 】
Lipase from Burkholderia cepacia immobilized on superparamagnetic nanoparticles using adsorption and chemisorption methodologies was efficiently applied as recyclable biocatalyst in the enzymatic kinetic resolution of (RS)-1-(phenyl)ethanols via transesterification reactions. (R)-Esters and the remaining (S)-alcohols were obtained with excellent enantiomeric excess (> 99%), which corresponds to a perfect process of enzymatic kinetic resolution (conversion 50%, E > 200). The transesterification reactions catalysed with B. cepacia lipase immobilized by the glutaraldehyde method showed the best results in terms of reusability, preserving the enzyme activity (conversion 50%, E > 200) for at least 8 successive cycles.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
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