Journal of the Brazilian Chemical Society | |
Enzymatic kinetic resolution of (RS)-1-(Phenyl)ethanols by Burkholderia cepacia lipase immobilized on magnetic nanoparticles | |
Netto, Caterina G. C. M.1  Andrade, Leandro H.1  Laboratory of Fine Chemistry and Biocatalysis1  Rebelo, Lya P.1  University of São Paulo, São Paulo, Brazil1  Toma, Henrique E.1  | |
关键词: superparamagnetic nanoparticles; lipase; transesterification catalysis; enantiomeric resolution; | |
DOI : 10.1590/S0103-50532010000800019 | |
学科分类:化学(综合) | |
来源: SciELO | |
【 摘 要 】
Lipase from Burkholderia cepacia immobilized on superparamagnetic nanoparticles using adsorption and chemisorption methodologies was efficiently applied as recyclable biocatalyst in the enzymatic kinetic resolution of (RS)-1-(phenyl)ethanols via transesterification reactions. (R)-Esters and the remaining (S)-alcohols were obtained with excellent enantiomeric excess (> 99%), which corresponds to a perfect process of enzymatic kinetic resolution (conversion 50%, E > 200). The transesterification reactions catalysed with B. cepacia lipase immobilized by the glutaraldehyde method showed the best results in terms of reusability, preserving the enzyme activity (conversion 50%, E > 200) for at least 8 successive cycles.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912050581088ZK.pdf | 790KB | download |