Journal of the Brazilian Chemical Society | |
Oxidative DNA damage induced by S(IV) in the presence of Cu(II) and Cu(I) complexes | |
María V. Alipázaga2  Giselle Cerchiaro1  Horácio D. Moya1  Nina Coichev2  | |
[1] ,Universidade de São Paulo Instituto de Química São Paulo SP ,Brazil | |
关键词: DNA damage; copper complexes; Cu(III); Cu(I); sulfite; | |
DOI : 10.1590/S0103-50532009000700015 | |
来源: SciELO | |
【 摘 要 】
The DNA damage induced by S(IV) in the presence of some Cu(II) complexes in air saturated solution was investigated. The addition of S(IV) to an air saturated solution containing CuII GGA (GGA = glycylglycyl-L-alanine), CuII G3 (G3 = triglycine) or CuII G4 (G4 = tetraglycine) and Ni(II) traces, causes rapid formation of the respective Cu(III) complex, with simultaneous O2 uptake and S(IV) oxidation. SO3•- and HO• were detected by EPR-spin trapping experiments. The DNA strand breaks were attributed to the oxysulfur radicals formed. In the reduction of Cu(II)/BCA (BCA = 4,4' dicarboxy-2-2'-biquinoline) by S(IV), with CuI BCA complex formation, there is the possible formation of carbon centered radical of BCA or peroxyl radical (ROO•) capable of oxidizing DNA bases. The intensity of DNA damage in the presence of these Cu(II) complexes and S(IV) (10-300 µmol L-1) followed the order: CuII BCA ∼ CuII G4 ∼ Cu(II) (added as Cu(NO3)2) > CuII G3 ∼ CuII GGA. Specifically for CuII BCA the damage occurred even at lower S(IV) concentration (0.1 µmol L-1). For the Cu(II) complexes with glycylglycylhistidine, glycylhistidylglycine, glycylhistidyllysine and glycylglycyltyrosylarginine the Cu(III) formation and the DNA damage was not observed.
【 授权许可】
CC BY
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