Electron transfer reactivity and the catalytic activity of hemoglobin incorporated in dimethylaminoethyl methacrylate film

Journal of the Brazilian Chemical Society

Hui Zhou¹  Rongwu Yang¹  Libin Shang¹  Zhiqiang Zhu¹  Genxi Li¹ 

[1] ,Nanjing University Department of Biochemistry and National Laboratory of Pharmaceutical Biotechnology Nanjing P. R. ,China

关键词: direct electrochemistry;    hemoglobin;    dimethylaminoethyl methacrylate;    electrocatalysis;   

DOI  :  10.1590/S0103-50532005000700017

来源: SciELO

【 摘 要 】

Hemoglobin (Hb) was incorporated in dimethylaminoethyl methacrylate (DMAEMA) to form a film and the film was modified on pyrolytic graphite (PG) electrode. UV-Vis spectra suggested that Hb in the film could keep its secondary structure. Consequently, a pair of stable, well-defined, and quasi-reversible cyclic voltammetric peaks could be observed with the formal potential at -206 mV (vs. saturated calomel electrode), characteristic of heme Fe(III)/Fe(II) redox couple of the protein. The apparent heterogeneous electron transfer rate constant and other electrochemical parameters were presented. The catalytic activity of Hb in DMAEMA films toward hydrogen peroxide was also investigated.

【 授权许可】

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