Application of Carbon-Microsphere-Modified Electrodes for Electrochemistry of Hemoglobin and Electrocatalytic Sensing of Trichloroacetic Acid

Sensors

Wen-Cheng Wang²  Li-Jun Yan²  Fan Shi²  Xue-Liang Niu²  Guo-Lei Huang²  Cai-Juan Zheng¹  Wei Sun²  Yu Lei²  Ashutosh Tiwari² 

[1] Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, China

关键词: hemoglobin;    carbon microsphere;    direct electrochemistry;    trichloroacetic acid;   

DOI  :  10.3390/s16010006

来源: mdpi

【 摘 要 】

By using the hydrothermal method, carbon microspheres (CMS) were fabricated and used for electrode modification. The characteristics of CMS were investigated using various techniques. The biocompatible sensing platform was built by immobilizing hemoglobin (Hb) on the micrometer-sized CMS-modified electrode with a layer of chitosan membrane. On the cyclic voltammogram, a couple of quasi-reversible cathodic and anodic peaks appeared, showing that direct electrochemistry of Hb with the working electrode was achieved. The catalytic reduction peak currents of the bioelectrode to trichloroacetic acid was established in the linear range of 2.0~70.0 mmol·L⁻¹ accompanied by a detection limit of 0.30 mmol·L⁻¹ (3σ). The modified electrode displayed favorable sensitivity, good reproducibility and stability, which suggests that CMS is promising for fabricating third-generation bioelectrochemical sensors.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.