Electron transfer reactivity and the catalytic activity of hemoglobin incorporated in dimethylaminoethyl methacrylate film

Journal of the Brazilian Chemical Society

Yang, Rongwu¹  Li, Genxi¹  Nanjing University, Nanjing, China¹  Shang, Libin¹  Zhu, Zhiqiang¹  Zhou, Hui¹ 

关键词: direct electrochemistry;    hemoglobin;    dimethylaminoethyl methacrylate;    electrocatalysis;   

DOI  :  10.1590/S0103-50532005000700017

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

Hemoglobin (Hb) was incorporated in dimethylaminoethyl methacrylate (DMAEMA) to form a film and the film was modified on pyrolytic graphite (PG) electrode. UV-Vis spectra suggested that Hb in the film could keep its secondary structure. Consequently, a pair of stable, well-defined, and quasi-reversible cyclic voltammetric peaks could be observed with the formal potential at –206 mV (vs. saturated calomel electrode), characteristic of heme Fe(III)/Fe(II) redox couple of the protein. The apparent heterogeneous electron transfer rate constant and other electrochemical parameters were presented. The catalytic activity of Hb in DMAEMA films toward hydrogen peroxide was also investigated.

【 授权许可】

Unknown