Acta Cirurgica Brasileira | |
Does a small size needle puncture cause intervertebral disc changes? | |
Ana Carolina Issy1  Vitor Castania1  Joao Walter Silveira1  Marcello Henrique Nogueira-barbosa1  Carlos Ernesto Garrido Salmon1  Elaine Del-bel1  Helton Luis Aparecido Defino1  | |
关键词: Intervertebral Disc Degeneration; Models; Animal; Rats; Wistar; | |
DOI : 10.1590/S0102-865020150080000009 | |
来源: SciELO | |
【 摘 要 】
PURPOSE:Small size needles have been regularly used for intradiscal injection of innocuous/potential therapeutic compounds in experimental conditions, but also in clinic procedures, such as discography. Our aim was to investigate if a 30-gauge needle could trigger observable changes on intact intervertebral discs. We compared these effects to those induced by a large size needle (21-gauge), a well-known intervertebral disc degenerative model based on needle puncture.METHODS:Coccygeal intervertebral discs (Co8-9) of adult male Wistar rats were punctured with a 21-gauge needle, while the coccygeal levels Co7-8 and Co9-10 remained intact. The 30-gauge needle was used to inject a safe volume of saline (2 µl) on both intact (Co9-10) and punctured (Co8-9) discs. MRI and histological score were performed at 2, 15 and 42 days after procedure.RESULTS: MRI analyses revealed significant reduction on signal intensity of 21-gauge punctured discs. Intact discs which received a saline injection through a 30-gauge needle also revealed significant alterations in the MRI signal when compared with control discs. No histological changes were observed in the intact saline injected discs at any time analyzed.CONCLUSION: Since significant intervertebral image changes were observed with a 30-gauge needle, cautious interpretation of the pharmacological inoculation findings is required.
【 授权许可】
CC BY
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