Closed head injury in rats: histopathological aspects in an experimental weight drop model

Acta Cirurgica Brasileira

Danilo Dos Santos Silva²  José Nazareno Pearce De Oliveira Brito¹  Jerúsia Oliveira Ibiapina¹  Marcel Fernando Miranda Batista Lima²  Andréa Ribeiro Gonçalves De Vasconcelos Medeiros²  Bárbara Hamedy Carvalho E Queiroz²  Aline Lariessy Campos Paiva²  Vanessa Olímpio De Melo Guedes² 

[1] ,UESPI Faculty of MedicinePiaui ,Brazil

关键词: Models;    Animal;    Brain Injuries;    Pathology;    Rats;    Modelos Animais;    Traumatismos Encefálicos;    Patologia;    Ratos;   

DOI  :  10.1590/S0102-86502012000400002

来源: SciELO

【 摘 要 】

PURPOSE: To study histopathological findings due to a model of closed head injury by weight loss in rats. METHODS: A platform was used to induce closed cranial lesion controlled by weight loss with a known and predefined energy. 25 male Wistar rats (Rattus novergicus albinus) were divided in five equal groups which received different cranial impact energy levels: G1, G2, G3 and G4 with 0.234J, 0.5J, 0.762J and 1J respectively and G5 (Sham). Under the effect of analgesia, the brain of each group was collected and prepared for histopathological analysis by conventional optic microscopy. RESULTS: It was observed greater number of injured neurons in animals of group 4, however neuronal death also could be noticed in animals of group 5. Intraparenchymal hemorrhages were more frequent in animals of group 4 and the cytotoxic brain swelling and vascular congestion were more intense in this group CONCLUSION: The histopathological analysis of these findings allowed to observe typical cranial trauma alterations and these keep close relation with impact energy.

【 授权许可】

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