Evaluation of lipoic acid topical application on rats skin wound healing

Acta Cirurgica Brasileira

Irene Clemes Külkamp-guerreiro²  Marielly Nunes Souza¹  Mariana Domingues Bianchin¹  Mateus Isoppo¹  Joana Sachetti Freitas¹  João Alex Alves¹  Anna Paula Piovezan¹  Adriana Raffin Pohlmann¹  Sílvia Stanisçuaski Guterres¹ 

[1] ,Federal University of Rio Grande do Sul Faculty of Pharmacy Graduate Studies Program in Pharmaceutical SciencesPorto Alegre RS ,Brazil

关键词: Thioctic Acid;    Wound Healing;    Nanocapsules;   

DOI  :  10.1590/S0102-86502013001000004

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the effects of lipoic acid (thioctic acid) topical application on wound healing on rats skin, and the consequences of lipoic acid nanoencapsulation on this process. METHODS: The model used was the healing activity on wounds induced by surgical incision on rats skin (n = 44). The parameters analyzed (11 days) were wound healing rate and histology (vascular proliferation, polymorphonuclear or mononuclear cells, and collagen synthesis or reepithelialization), after application of free lipoic acid or lipoic acid- loaded nanocapsules. The antioxidant activity of these formulations was evaluated by lipid peroxidation test. RESULTS: It was demonstrated for the first time that the topical application of lipoic acid improves wound healing. On the seventh day after surgery, the animals treated with lipoic acid showed increased healing rate (60.7 ± 8.4%) compared to the negative control group (43.0 ± 17.4%), as so improvement of histological parameters. The nanoencapsulation reverted the pro-oxidant activity presented in vitro by lipoic acid, whereas diminished wound repair. CONCLUSIONS: The topical application of lipoic acid produced an increase in the skin wound healing, which may be related to its pro-oxidant activity. On the other hand, the nanoencapsulation of the lipoic acid reversed the pro-oxidant activity, although presented minor healing activity.

【 授权许可】

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