Does alfa lipoic acid prevent liver from methotrexate induced oxidative injury in rats?

Acta Cirurgica Brasileira

Tuğrul Çakır¹  Ahmet Baştürk¹  Cemal Polat¹  Arif Aslaner¹  Himmet Durgut¹  Ahmet Özer Şehirli¹  Mehmet Gül¹  Ayliz Velioğlu Öğünç¹  Semir Gül¹  Mehmet Zafer Sabuncuoglu¹  Mehmet Tahir Oruç¹ 

关键词: Thioctic Acid;    Methotrexate;    Liver;    Rats;   

DOI  :  10.1590/S0102-865020150040000003

来源: SciELO

【 摘 要 】

PURPOSE: To determine the antioxidant and anti-inflammatory effects of alfa lipoic acid (ALA) on the liver injury induced by methotrexate (MTX) in rats.METHODS:Thirty two rats were randomly assigned into four equal groups; control, ALA, MTX and MTX with ALA groups. Liver injury was performed with a single dose of MTX (20 mg/kg) to groups 3 and 4. The ALA was administered intraperitonealy for five days in groups 2 and 4. The other rats received saline injection. At the sixth day the rats decapitated, blood and liver tissue samples were removed for TNF-α, IL-1β, malondialdehyde, glutathione, myeloperoxidase and sodium potassium-adenosine triphosphatase levels measurement and histological examination.RESULTS: MTX administration caused a significant decrease in tissue GSH, and tissue Na+, K+ ATPase activity and which was accompanied with significant increases in tissue MDA and MPO activity. Moreover the pro-inflammatory cytokines (TNF-α, IL- β) were significantly increased in the MTX group. On the other hand, ALA treatment reversed all these biochemical indices as well as histopathological alterations induced by MTX.CONCLUSION:Alfa lipoic acid ameliorates methotrexate induced oxidative damage of liver in rats with its anti-inflammatory and antioxidant effects.

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