Cenostigma macrophyllum Tul. on the healing of skin wounds in rats with Diabetes mellitus

Acta Cirurgica Brasileira

Nayana Pinheiro Machado De Freitas Coelho²  Veruska Cronemberger Nogueira²  Maria Angélica Gargione Cardoso¹  Luciano Da Silva Lopes¹  Paulo Pedro Do Nascimento¹  Evair Dos Santos Rocha¹  Caio Leôncio Probo Da Silva¹  Emília Ângela Loschiavo Arisawa¹ 

[1] ,UNIVAP Postgraduate Program in Biomedical Engineering Sao Jose dos Campos SP ,Brazil

关键词: Diabetes mellitus;    Plants;    Medicinal;    Wound Healing;    Nitric Oxide;    Skin;    Rats;   

DOI  :  10.1590/S0102-86502013000800007

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the effect of Cenostigma macrophyllum Tul. in the tissue repair process of skin lesions in rats with induced Diabetes mellitus. METHODS: Sixty-three male Wistar rats were distributed into three groups: control (C), diabetic (D), and diabetic treated with an oil-in-water emulsion of the plant (DPL) subdivided according to time of observation (seven, 14, and 28 days). Diabetes was induced by administration of by injection of streptozotocin (50 mg/kg, after a 12-h fast) into the penile vein and confirmed by glucose levels exceeding 240 mg/dL. Held surgical lesion (2.5 cm in diameter) on the back of the animals. The DPL received topical application of group of the oil-in-water emulsion plant (0.5ml). To evaluate the levels of nitric oxide, was collected 5ml of blood from the abdominal aorta, after his euthanasia. The samples of interest were sent for routine histological processing. RESULTS:A reduction in the percentage of inflammatory cells and increased numbers of fibroblasts in the group DPL, seven days, compared to the other groups. At 14 days, the DPL group also showed a higher concentration of nitric oxide (p<0.01) than in groups C and D. CONCLUSION: The oil-in-water emulsion of C. macrophyllum Tul accelerated wound healing in diabetic rats.

【 授权许可】

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