Acta Cirurgica Brasileira | |
Oxidative stress gene expression profile in inbred mouse after ischemia/reperfusion small bowel injury | |
Paulo Roberto Bertoletto2  Adauto Tsutomu Ikejiri2  Frederico Somaio Neto2  José Carlos Chaves2  Roberto Teruya1  Eduardo Rodrigues Bertoletto1  Murched Omar Taha1  Djalma José Fagundes1  | |
[1] ,UFGD Medical School Dourados MS ,Brazil | |
关键词: Oxidative Stress; Antioxidants; Gene expression; Ischemia; Reperfusion; Real-Time Polymerase Chain Reaction; Mice; Estresse Oxidativo; Antioxidantes; Expressão Gênica; Isquemia; Reperfusão; Reação em Cadeia da Polimerase em Tempo Real; Camundongos; | |
DOI : 10.1590/S0102-86502012001100006 | |
来源: SciELO | |
【 摘 要 】
PURPOSE: To determine the profile of gene expressions associated with oxidative stress and thereby contribute to establish parameters about the role of enzyme clusters related to the ischemia/reperfusion intestinal injury. METHODS: Twelve male inbred mice (C57BL/6) were randomly assigned: Control Group (CG) submitted to anesthesia, laparotomy and observed by 120min; Ischemia/reperfusion Group (IRG) submitted to anesthesia, laparotomy, 60min of small bowel ischemia and 60min of reperfusion. A pool of six samples was submitted to the qPCR-RT protocol (six clusters) for mouse oxidative stress and antioxidant defense pathways. RESULTS: On the 84 genes investigated, 64 (76.2%) had statistic significant expression and 20 (23.8%) showed no statistical difference to the control group. From these 64 significantly expressed genes, 60 (93.7%) were up-regulated and 04 (6.3%) were down-regulated. From the group with no statistical significantly expression, 12 genes were up-regulated and 8 genes were down-regulated. Surprisingly, 37 (44.04%) showed a higher than threefold up-regulation and then arbitrarily the values was considered as a very significant. Thus, 37 genes (44.04%) were expressed very significantly up-regulated. The remained 47 (55.9%) genes were up-regulated less than three folds (35 genes - 41.6%) or down-regulated less than three folds (12 genes - 14.3%). CONCLUSION: The intestinal ischemia and reperfusion promote a global hyper-expression profile of six different clusters genes related to antioxidant defense and oxidative stress.
【 授权许可】
CC BY
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