L-Alanyl-Glutamine dipeptide pretreatment attenuates ischemia-reperfusion injury in rat testis

Acta Cirurgica Brasileira

João Paulo De Vasconcelos Leitão²  Jefferson Menezes Viana Santos¹  Raquel Cavalcante De Vasconcelos¹  José Huygens Parente Garcia¹  Paulo Roberto Leitão De Vasconcelos¹  Sergio Botelho Guimarães¹ 

[1] ,UFC Department of SurgeryCeara ,Brazil

关键词: Oxidative Stress;    Glutamine;    Testis;    Ischemia;    Reperfusion;    Rats;    Estresse Oxidativo;    Glutamina;    Testículo;    Isquemia;    Reperfusão;    Ratos;   

DOI  :  10.1590/S0102-86502011000700005

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the effect of alanyl-glutamine dipeptide (L-Ala-Gln) pre-treatment on ischemia-reperfusion (I/R) injury after unilateral testicular torsion-detorsion in a comparative controlled experiment. METHODS: Forty-eight rats (150-200 g) randomly distributed into 4 groups (n=12), and distributed in 2 subgroups (n=6) each, were treated with saline 2.0 ml (G-1, G-3) or L-Ala-Gln 20%, 0.75g/kg dissolved in saline (total volume 2.0 ml) administered in the left saphenous vein 30 minutes before ischemia. Anesthetized rats were subjected to I/R induced by torsion (720°) of the right spermatic cord lasting 1h (G-1, G-2) or 3 hours (G-3, G4). Anesthesia was again applied at the end of ischemia time (T-0) for testis detorsion and 6 hours later (T-6) for orchiectomy. All operations were performed on the right testes through transverse scrotal incisions. Right orchiectomy was carried out at the end of ischemia (T-0), and 6 hours later (T-6) to evaluate the concentrations of malondialdehyde (MDA) and reduced glutathione (GSH) in the testis. RESULTS: Pretreatment with L-Ala-Gln reduced MDA contents in rat testis at the end of ischemia lasting 3 hours. There was significant increase of GSH levels in T-6 time-point after 1 hour of ischemia. GSH levels also increased in T-0 and T-6 time-points in rats subjected to ischemia for 3 hours. CONCLUSION: L-Ala-Gln administered before torsion/detorsion of the spermatic cord decreases lipid peroxidation during ischemia and protects the testis from oxidative stress by upregulating GSH levels during reperfusion.

【 授权许可】

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