Propofol and N-Acetylcysteine attenuate oxidative stress induced by intestinal ischemia/reperfusion in rats: Protein carbonyl detection by immunoblotting

Acta Cirurgica Brasileira

Marcelo Aragão Insuellas De Azeredo²  Luciana Aragão Insuellas De Azeredo¹  Elis Cristina Araújo Eleuthério¹  Alberto Schanaider¹ 

[1] ,UFRJ Faculty of Medicine Rio de Janeiro,Brazil

关键词: Propofol;    Acetylcysteine;    Drug Synergism;    Oxidative Stress;    Ischemia;    Reperfusion;    Protein Carbonylation;    Immunoblotting;    Propofol;    Acetilcisteína;    Sinergismo Farmacológico;    Estresse Oxidativo;    Isquemia;    Reperfusão;    Carbonilação Protéica;    Immunoblotting;   

DOI  :  10.1590/S0102-86502008000500006

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the antioxidant effect of Propofol and N-Acetylcysteine (NAC) on intestinal ischemia/reperfusion (I/R) in rats by determining carbonyl protein level. METHODS: Forty Wistar rats were randomly assigned into the following groups: Control; Sham; I/R with Propofol; I/R with Propofol and NAC; I/R with Ketamine and Xylazine. The I/R groups underwent 60 minutes of ischemia and an equal period of reperfusion. Blood samples, collected by cardiac punction, were centrifuged for plasma obtainment. Protein carbonyl level in plasma samples was determined by immunoblotting. RESULTS: No significant difference in protein carbonyl level was found between Control and Sham groups (P>0.05). The highest reduction in protein carbonyl level (P<0.05) was obtained with the administration of Propofol and NAC (Group 4) in intestinal I/R procedure. CONCLUSION: The administration of Propofol and NAC showed the best antioxidant effect on oxidative stress in rats that underwent intestinal I/R procedure, suggesting a synergistic interaction.

【 授权许可】

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