Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats

Acta Cirurgica Brasileira

Paulo Fernandes Saad¹  Karen Ruggeri Saad²  Luiz Dantas De Oliveira Filho¹  Sueli Gomes Ferreira¹  Marcia Kiyomi Koike¹  Edna Frasson De Souza Montero¹ 

[1] ,Sao Francisco Valley Federal University Faculty of Medicine

关键词: Acetylcysteine;    Lung;    Shock;    Hemorrhagic;    Cell Death;    Rats;    Acetilcisteína;    Pulmão;    Choque Hemorrágico;    Morte Celular;    Ratos;   

DOI  :  10.1590/S0102-86502012000800008

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the effect of N-acetylcysteine (NAC) combined with fluid resuscitation on pulmonary cell death in rats induced with controlled hemorrhagic shock (HS). METHODS: Two arteries (MAP calculation and exsanguination) and one vein (treatments) were catheterized in 22 anesthetized rats. Two groups of male albino rats were induced with controlled HS at 35mmHg MAP for 60 min. After this period, the RL group was resuscitated with Ringer's lactate and the RL+NAC group was resuscitated with Ringer's lactate combined with 150mg/Kg NAC. The control group animals were cannulated only. The animals were euthanized after 120 min of fluid resuscitation. Lung tissue samples were collected to evaluate the following: histopathology, TUNEL and imunohistochemical expression of caspase 3. RESULTS: RL showed a greater number of cells stained by TUNEL than RL + NAC, but there was no change in caspase 3 expression in any group. CONCLUSION: N-acetylcysteine associate to fluid resuscitation, after hemorrhagic shock, decreased cell death attenuating lung injury.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License