Renal histology and immunohistochemistry after acute hemorrhage in rats under sevoflurane and ketoprofen effect

Acta Cirurgica Brasileira

Francisco Sobreira Guedes Jr¹  Deyvid Santos Da Cruz¹  Marcela Marcondes Pinto Rodrigues¹  Leopoldo Muniz Da Silva¹  Renée Laufer Amorim²  Pedro Thadeu Galvão Vianna²  Yara Marcondes Machado Castiglia² 

[1],UNESP

关键词: Ketoprofen;    Hemorrhage;    Kidney;    Cytokines;    Necrosis;    Rats;    Cetoprofeno;    Hemorragia;    Rim;    Citocinas;    Necrose;    Ratos;   

DOI  :  10.1590/S0102-86502012000100007

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the influence of intravenous nonselective cyclooxygenase inhibitor, ketoprofen (keto), on kidney histological changes and kidney cytokines, tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1), levels after hemorrhage of 30% of volemia (three times 10%, intervals of 10 min) in rats. METHODS: Under sevoflurane (sevo) anesthesia, sevo and sevo+keto groups (10 rats each) were instrumented for Ringer solution (5mL/kg/h) administration and mean arterial pressure (MAP) evaluation, plus keto (1.5mg/kg) administration in sevo+keto group in the beginning of anesthesia. Rectal temperature was continuously measured. The baseline data of temperature and MAP were collected at the first hemorrhage (T1), the third hemorrhage (T2) and 30min after T2 (T3). Bilateral nephrectomy was achieved for histology and immunohistochemistry. RESULTS: In both groups, temperature and MAP diminished from initial values. Hypothermia was greater in sevo group (p=0.0002). Tubular necrosis was more frequent in sevo group (p=0.02). The studied cytokines were equally present in the kidneys of both groups. CONCLUSION: Ketoprofen was more protective to the rat kidney in condition of anesthesia with sevoflurane and hypovolemia, but it seems that TNF-α and IL-1 were not involved in that protection.

【 授权许可】

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