Experimental model of smoking and simulation of reflux with acid and pepsin in rats

Acta Cirurgica Brasileira

José Hélio Zen Junior¹  André Del Negro¹  José Alexandre Colli Neto¹  Marina Rachel Araujo¹  Albina Maria Altemani²  Nelson Adami Andreollo² 

[1] ,UNICAMPCampinas SP ,Brazil

关键词: Smoking;    Hydrochloric Acid;    Pepsin;    Gastroesophageal Reflux;    Rats;    Tabagismo;    Ácido Clorídrico;    Pepsina;    Refluxo Gastroesofágico;    Ratos;   

DOI  :  10.1590/S0102-86502012000100004

来源: SciELO

【 摘 要 】

PURPOSE: To develop experimental models to evaluate the effects of hydrochloric acid associated with the pepsin instilled in the mucosa of the upper esophagus and the esophagogastric junction of young male rats Wistar, simulating injury caused by gastroesophageal reflux on the mucosa of aero-digestive tract in humans as well as the action of the risk exposure of mucosa to cigarette smoke. METHODS: Fifty young male Wistar rats divided in 5 groups with 10 animals each one, respectively simulating pharyngo-laryngeal reflux and gastroesophageal reflux, pharyngo-laryngeal reflux and smoking, smoking only, gastroesophageal reflux and control group. RESULTS: The histopathologic studies no recorded neoplasias, only mild changes and no significant alterations. The hemo-oximetry (carboxyhemoglobin and methemoglobim) and CO2 concentration confirm that the animals were submitted to high intensity of exposure to carcinogens in tobacco and its derivatives. CONCLUSION: The experimental models were highly efficient, practical, easy to use and economical and can be employed in other similar studies to determine the harmful effects by smoking and reflux.

【 授权许可】

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